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Image Search Results


KEY RESOURCES TABLE

Journal: Neuron

Article Title: CRISPR interference-based platform for multimodal genetic screens in human iPSC-derived neurons

doi: 10.1016/j.neuron.2019.07.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat polyclonal anti-human progranulin R&D Systems Cat#AF2420; RRID:AB_2114489 Mouse monoclonal anti-TUJ1 BioLegend Cat#801201; RRID:AB_2313773 Donkey polyclonal anti-goat AF-488 Jackson ImmunoResearch Cat#705-545-147; RRID:AB_2336933 Donkey polyclonal anti-mouse RRX Jackson ImmunoResearch Cat#715-295-151; RRID:AB_2340832 Donkey polyclonal anti-goat AF-647 Jackson ImmunoResearch Cat#705-605-147; RRID:AB_2340437 Donkey polyclonal anti-mouse AF-488 Jackson ImmunoResearch Cat#715-545-151; RRID:AB_2341099 Guinea pig polyclonal anti-NeuN MilliporeSigma Cat#ABN90; RRID:AB_11205592 Mouse monoclonal anti-human tau ThermoFisher Scientific Cat#MN1000; RRID:AB_2314654 Goat polyclonal anti-guinea pig AF-647 ThermoFisher Scientific Cat#A-21450; RRID:AB_2735091 Mouse monoclonal anti- β-Actin (8H10D10) Cell Signaling Technology Cat# 3700, RRID:AB_2242334 Mouse monoclonal anti- phospho-Histone H2A.X (Ser139) Millipore Cat# 05-636, RRID:AB_309864 Rabbit monoclonal anti-UBQLN2 (D7R2Z) Cell Signaling Technology Cat# 85509, RRID:AB_2800056 IRDye 680RD Goat anti-Mouse IgG LI-COR Biosciences Cat# 926-68070, RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat# 926-32211, RRID:AB_621843 Chemicals, Peptides, and Recombinant Proteins Trimethoprim Sigma Cat#92131-5G CNQX disodium salt Tocris Cat#1045 Puromycin dihydrochloride Sigma Cat#P9620-10ML Blastidicin S HCl Gemini Bio-Products Cat#400-165P Hoechst 33342 ThermoFisher Scientific Cat#62249 DRAQ5 ThermoFisher Scientific Cat#62251 Lipofectamine Stem ThermoFisher Scientific Cat#STEM00003 Alt-R S.p.

Techniques: Recombinant, Transfection, Knock-Out, Multiplex Assay, RNA HS Assay, Plasmid Preparation, Software